Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 257, Issue -, Pages 734-744Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.10.172
Keywords
Amphiphilic thiophene copolymers; Quantum dots; Heparin; Spermine; Fluorescent turn off-on
Funding
- Korea Research Fellowship program - Ministry of Science and ICT [NRF-2015H1D3A1066235]
- National Research Foundation of Korea [NRF-2017R1D1A3B03035689, NRF-2017R1A2B4006388]
Ask authors/readers for more resources
Novel turn off-on optosensors based on amphiphilic thiophene copolymers coated CdTe quantum dots (P1QDs and P2QDs) were developed for the dual detection of heparin and spermine. The emission of the P1QDs and P2QDs is found to be quenched in the presence of heparin by electron transfer mechanism through electrostatic and hydrogen bonding interactions. Upon the addition of spermine, heparin has been removed from the surface of P1QDs and P2QDs due to the strong electrostatic interactions between spermine and heparin, which showed significant fluorescence recovery of the sensors. Under optimum conditions, both P1QDs and P2QDs sensors displayed excellent limits of detection for heparin of 1.59 nmol L-1 (R-2 = 0.9955) and 0.88 nmol L-1 (R-2 = 0.9908) with linear ranges of 1-11 mu mol L-1 and 1-10 mu mol L-1, respectively. P1QDs and P2QDs also showed remarkable limits of detection for spermine of 2.90 nmol L-1 (R-2 = 0.9957) and 1.66 nmol L-1 (R-2 = 0.9928) with linear ranges of 0.05-15 mu mol L-1 and 1-12 mu mol L-1, respectively. The P1QDs and P2QDs sensors show a high selectivity to heparin and spermine over common interfering substances and ions. In addition, the proposed method was successfully utilized in serum samples, which could facilitate the further heparin and spermine biochemical and biomedical research. (c) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available