Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 265, Issue -, Pages 217-226Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.03.042
Keywords
Carbon nanofibers; Mesoporous carbon-gold nanoparticles; Screen-printed carbon electrodes; Aptasensor; Kanamycin; Streptomycin
Funding
- National Natural Science Foundation of China [31471641, 31772068, 31701681]
- Special Project of Independent Innovation of Shandong Province [2014CGZH0703]
- Shandong Provincial Natural Science Foundation [ZR2014CM009, ZR2015CM016, ZR2016CM29, ZR2017BC001, ZR2014FL003]
- Key Research and Invention Program of Shandong Province [2017GNC10119]
- Key Innovative project for Major Agriculture Application Technology of Shandong Province
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We proposed a multiplex electrochemical aptasensor based on a screen-printed carbon electrode (SPCE), which was modified by carbon nanofibers (CNFs) and mesoporous carbon-gold nanoparticles (OMC-AuNPs) for the ultrasensitive detection of kanamycin (KAN) and streptomycin (STR). The designed aptasensor had some characteristics of a high electrochemical conductivity and a high specific surface area because of rod-like OMC-AuNPs and CNFs. Moreover, CNFs and OMC-AuNPs could be homogeneously and firmly adhered to the surface of SPCE, and complimentary strands of aptamers could also be well immobilized on the surface of the electrode modified with CNFs and OMC-AuNPs. In the absence of KAN and STR, the aptamers bound to their complimentary strands. Upon addition of KAN and STR, the aptamers bound to their targets, which led to the complementary strands released from the aptamers and more changes of current peaks because of the aptamers labelled with CdS and PbS. Under the optimal conditions, this aptasensor showed a high stability and a selectivity toward KAN and STR with limits of detection (LODs) as low as 87.3 and 45.0 pM, respectively. The applicability of the developed aptasensor was successfully assessed by detection of KAN and STR in a spiked milk sample without any interference from the sample matrix. It is expected that the proposed aptasensor can be easily detect residues of other antibiotics in milk by replacing different aptamers. (C) 2018 Elsevier B.V. All rights reserved.
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