Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 271, Issue -, Pages 110-117Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.05.119
Keywords
AlGaN/GaN HEMT; Electrical double layer; Sensitivity and specificity of DNA detection
Funding
- Ministry of Science Technology [MOST 105-2221-E-007-008-]
- National Tsing Hua University [106N523CE1]
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In this research, we have realized an electrical double layer (EDL) gated high electron mobility transistor (HEMT) as DNA sensor. The sensing area on the gate electrode which is separated from the transistor channel is immobilized with probe DNA to capture target DNA from physiological salt environment. The detection limit of the sensor can be as low as 1 fM with very high sensitivity. The specificity of the DNA sensor is also demonstrated by controlling the hybridization temperature. By choosing the hybridization temperature slightly lower than the melting temperature of the well-matched sequence, the binding ratio can be controlled between the fully-matched and mismatched one. The sensor has demonstrated specificity, with the ability to achieve single base mismatch resolution. The sensor has the potential for rapid DNA sensing applications in cells, biomarkers and viruses.
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