Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 256, Issue -, Pages 89-97Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.10.049
Keywords
Aptamer; CA125; MUC16; Ovarian cancer; CNT; Immunoassay
Funding
- National Research Foundation (NRF) - Korean government (MSIP) [2012M3A7B4035286, 2016M3A9B4918833]
- MSIT (Ministry of Science and ICT), Korea under ITRC (Information Technology Research Center) [IITP-2017-1711055214]
- NRF - Ministry of Education [2012R1A6A1029029]
- AFOSR/AOARD, USA [FA2386-15-1-4081]
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Despite the major role of cancer antigen 125 (CA125) in cancer progression, its structural diversity makes antibody-based immunoassay of this protein difficult. Here we report the selection of an anti-CA125 ssDNA aptamer and its application to sensitive detection of CA125 when used together with a CA125-specific antibody immobilized on a three-dimensional network of carbon nanotubes (3DN-CNTs). With exceptional stability and easy modifications over antibodies, the selected ssDNA aptamer (rCAA-8) showed high binding affinity (166 nM) for recombinant CA125, which enabled CA125-specific imaging in ovarian cancer cells (OVCAR-3). Furthermore, when compared to other fluorescent assays based on graphene oxide or flat surface and traditional enzyme-linked immunosorbent assay, a chip-based assay using a 3DN-CNT surface and anti-CA125 antibody-aptamer pair resulted in higher sensitivity and broader dynamic range as a function of CA125 concentration, due to high target specificity and high surface loading density. Our on-chip aptamer-based assay will facilitate sensitive and specific monitoring of CA125 in the biological and clinical fields. (C) 2017 Elsevier B.V. All rights reserved.
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