Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 261, Issue -, Pages 210-217Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.01.132
Keywords
Lysosomal micropolarity; Ratiometric fluorescent probe; Cancer cell apoptosis; Real-time estimating; Ultrasensitive
Funding
- National Natural Science Foundation of China [21402043, 21722501]
- Program for Science Technology Innovation Talents and Team in Universities of Henan Province [18HASTIT001, 18IRTSTHN002]
- Key Project of Science and Technology of Henan Province [162102210267]
- Key Scientific Research Project of Higher Education of Henan Province [16B150004, 18A150046]
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Lysosomal micropolarity-change is one of the ultimate reflection factors of cell apoptosis and death in complex living organism. Thus, rapid and sensitive detecting of lysosomal micropolarity-change is beneficial for real-time estimating of cancer cell apoptosis. Many polarity-specific fluorescent probes have been reported, but lysosomal micropolarity-ultrasensitive ratiometric fluorescent probe with excellent two-photon property for real-time estimating the cancer cell apoptosis is scarce. In this work, an intramolecular charge transfer (ICT) two-photon ratiometric fluorescent probe (DC) was reported and evaluated to real-time estimate cancer cell apoptosis through ultrasensitive detection of lysosomal micro-polarity. DC appeared different fluorescence intensities at 580 and 600 nm in cancer cells, specifically. And its fluorescence change presented a linear change on ratio mode in the range of polarity (Delta f=0.2230-0.3274). The lowest detection of DC for polarity change can as low as Delta f=0.018 by fluorescent ratiometric signals. More importantly, based on the ultrasensitive changes of lysosomal micro-polarity, DC can be used to real-time estimate cancer cell apoptosis by microscopic imaging and flow cytometry. Thus, DC may have potential application varying from detecting of lysosomal micropolarity changes to real-time estimating of cancer cell apoptosis. (C) 2018 Elsevier B.V. All rights reserved.
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