Carbon paste electrode based on functional GOx/silica-lignin system to prepare an amperometric glucose biosensor

In this paper, a novel procedure for receiving enzyme biosensor based on the cheap and functional silica/ lignin (SiO2/Lig) hybrid material is presented. In this study, a functional biohybrid SiO2/Lig was selected to conduct the immobilization of glucose oxidase (GOx) by adsorption on its surface. The immobilized amount of GOx at SiO2/Lig was 25.28 mg g(-1), twice as much compared to its amount at non-functionalized SiO2. The system of GOx-SiO2/Lig was combined with single-walled carbon nanotubes/ platinum nanoparticles support to prepare a I generation glucose biosensor. Moreover, the GOx-SiO2/ Lig based carbon paste electrode with ferrocene redox mediator was evaluated as an active material in II generation glucose biosensor. The GOx-SiO2/Lig/CPE was subjected to an examination in glucose solution by electrochemical techniques such as cyclic voltammetry (CV) and chronoamperometry. The obtained results suggest that GOx-SiO2/Lig can be a material of choice for preparation of an efficient and low-cost biosensor working in various electrode configurations. The newly obtained glucose biosensor presents promising electrochemical parameters for glucose determination. The glucose-sensing sensitivity amounted 0.78 mu A mM(-1). The biosensor showed a linear response range of 0.5-9 mM with a detection limit (LOD) of 145 mu M. (C) 2017 Elsevier B.V. All rights reserved.