Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 261, Issue -, Pages 58-65Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.01.131
Keywords
Fluorescent oligonucleotide probe; Lead (II) ions; Detection; Silver (I) ions; Water; Food
Funding
- Drug Discovery Initiative in the Major Natural Science and Technology Project of China [2009X09103-696]
- National Natural Science Foundation of China [21075097]
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Owing to the high toxicity of lead (II) (Pb2+) and silver (I) ions (Ag+) to aquicolous organisms, it is highly desirable to develop a sensitive method for the simultaneous detection of Pb2+ and Ag+. In this work, a novel and sensitive single-labeled fluorescent oligonucleotide (OND) probe is designed to simultaneously detect based on the specific complexation of Ag+ to cytosines, the special induction capacity of Pb2+ to guanine-rich OND to form G-quadruplex and the inherent quenching ability of G-quadruplex to the hexachloro fluorescein (HEX). The Ag+-induced hairpin-link structure makes HEX labeled at the 5'-termini close to the Pb2+-induced G-quadruplex connected at the T-terminin, resulting in a remarkable fluorescence quenching owing to photoinduced electron transfer (PET) process from G-quadruplex to HEX. At 298 K, the apparent associating constant between them is 1.85 x 10(8) (L/mol). Using this system, we can simultaneously detect the Pb2+ and Ag+ over the linear response range of 1-100 nmol/L with a detection limits of 96 pmol/L and 21 pmol/L, respectively. Through the assay in real samples, it is known that this system could be applied to accurately monitor the trace Pb2+ and Ag+ in aqueous solution. (C) 2018 Elsevier B.V. All rights reserved.
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