Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 260, Issue -, Pages 295-302Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.01.013
Keywords
Fluorescein; Fluorescent probe; Glutathione; Cysteine; Cell imaging
Funding
- National Natural Science Foundation of China [21504089, U1304202]
- Scientific and Technological Projects of Henan province [162300410209, 152102210343, 162300410236]
- Program for Science and Technology Innovation Talents in Universities of Henan Province [15HASTIT002]
- key project of education department of Henan Province [17A150050]
- Key Program in Universities of Henan Province [15B150016]
- Scientific and Technological Projects of Xuchang
- National Students' Platform for Innovation and Entrepreneurship Training Program [201710480001]
Ask authors/readers for more resources
A new fluorescein-based probe (Py-F) was designed and synthesized to develop an optical and fluorescent sensor for biological thiols. Py-F possessed the thiol-responsed disulfide group and displayed selectivity and sensitivity for glutathione (GSH) and cysteine (Cys) over homocysteine (Hcy) in the CH3CN/PBS buffer solution, and the fluorescence detection limit was calculated to be 0.12 mu M and 0.13 mu M, respectively. Besides, the recognition mechanism was explained by density functional theory (DFT) calculation, and confirmed by mass spectroscope measurement. Furthermore, the thiols detection application in cellular imaging assays indicated that the probe has appreciable cell permeability and can serve as a fluorescent probe for detecting biothiols in living cells. (c) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available