Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 255, Issue -, Pages 1726-1734Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.08.188
Keywords
Aggregation induced emission; Lectin recognition; E.coli detection; Glycoacrylamides; Fluorescence
Ask authors/readers for more resources
Development of fluorescent glycopolymeric probes with high water solubility, multivalency, stimuli responsiveness, high cellular internalization efficiency and good photostability, is in urgent demand. Aggregation Induced Emission (AIE) property of glucose based acrylamides (Glc-acryl and Glc-bis) have been studied at various pH, concentration and their application towards lectin recognition, bacterial detection and cell imaging have been probed. The emissions of glycoacrylamides are found to be pH dependent and exhibit Aggregation Induced Emission Enhancement (AIEE) in t-butanol-water mixtures. Fluorescent assay unravelled the specific recognition property of pendant glucose residues on glycoacrylamides towards protein, Concanavalin A (Con A), with an association constant (K-a) of 144 x 10(3) M-1 and 76.3 x 10(3) M-1, for the interaction with Glc-acryl and Glc-bis, respectively. The limit of detection (LOD) of Con A by Glc-acryl and Glc-bis was found to be 7.85 pM and 3.49 nM, respectively. Incubation with E.coli revealed that the relationship between the fluorescence signal and E.coli concentration was linear from (1.00 x 10(6)) to (1.70 x 10(8)) cells/mL (R-2 = 0.98) with LOD (at S/N ratio of 3) of E.coli being 7.30 x 10(5) cells/mL. The cell penetration studies of synthesized glycoacrylamides with INT407 cell lines showed good cellular uptake. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available