Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 261, Issue -, Pages 233-240Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.01.130
Keywords
AIE; Tetraphenylbenzene; Fluorescent probe; Protamine
Funding
- National Natural Science Foundation of China [21773140]
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An amphiphilic aggregation-induced emission (AIE) tetraphenylbenzene derivative, namely 6(1,3-dioxo-4,5,6,7-tetraphenylisoindolin-2-yl) hexanoic acid (TPHA), was designed. TPHA exhibits pH-responsive AIE properties in aqueous solutions. Its fluorescence intensity in dispersed systems of acidic THE/water increased with an increasing volume ratio of water due to the formation of aggregates, while only very weak fluorescence was observed in basic THE/water dispersions. The formation of aggregates in acidic dispersions could be attributed to the hydrophobic effect, intermolecular pi-pi interaction, and possible hydrogen bonding between the carboxyl groups. Deprotonation of the carboxyl moieties in basic dispersions resulted in the formation of molecularly dispersed systems. The fluorescence intensity of the dispersions in basic media increased dramatically (turn-on signals) when adding protamine, due to the formation of aggregates via electrostatic interactions between the deprotonated carboxyl groups and protein molecules. A good linear relationship between the fluorescence intensities and the protein concentrations in the range of 0-6 mu g/mL was obtained, and the detection limit was calculated to be 4.78 ng/mL. In addition, heparin was found to quench the fluorescence (turn-off signals) of the TPHA-protamine system effectively. Therefore, this strategy can be used as fluorescence probes for both protamine and heparin detection. (C) 2018 Elsevier B.V. All rights reserved.
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