Disposable electrochemical aptasensor based on carbon nanotubes-V2O5-chitosan nanocomposite for detection of ciprofloxacin

A novel electrochemical aptasensing transducer platform has been developed based on carbon nanotube (CNT)-V2O5-chitosan (CS) nanocomposites modified screen printed carbon electrode (SPCE) for the detection of ciprofloxacin. Using CNT-V2O5-CS/SPCE as the basal electrode transducer platform for the immobilization of the single-stranded DNA aptamer, and the electrochemical impedance spectroscopy (EIS) was selected for quantitative detection of ciprofloxacin (CIP). This aptasensor combines the bio-compatibility of V2O5 nanoparticles, the efficient electron transfer capability of multiwalled CNTs, the effective film-forming strength of CS and the portability of SPCE. Under optimized experimental parameters, the developed aptasensor exhibited a dynamic range from 0.5 to 64.0 ng mL(-1), where the linearity was between 0.5 to 8.0 ng mL(-1) and the limit of detection (LOD) was 0.5 ng mL(-1) of CIP. Furthermore, it showed good selectivity to CIP. For practical application, the aptasensor performance was verified in spiked milk samples and the acceptable recovery of 94.50-97.87% (%RSD = 4.38, n = 3) was obtained. (c) 2018 Elsevier B.V. All rights reserved.