Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 265, Issue -, Pages 339-345Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.03.083
Keywords
Acrylamide; Biosensor; Fluorescence intensity; Gold nanoparticles; Potato fries
Funding
- Mashhad University of Medical Sciences
- Sari Agricultural Sciences & Natural Resources University (SANRU)
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To protect human health from the side effects of acrylamide in heat-processed food samples, simple analytical approaches are highly desired to determine low concentrations of acrylamide. In this study, a simple, rapid and accurate fluorescent sensor was developed for detection of acrylamide based on gold nanoparticles (AuNPs) and FAM-labeled double-stranded DNA (FAM-dsDNA). The sensing method was developed in a way to produce a remarkable fluorescence intensity difference in the absence and presence of acrylamide. In the presence of acrylamide, the single-stranded DNA (ssDNA) and acrylamide adduct is formed. So that, FAM-labeled complementary strand DNA (FAM-csDNA) is free in the environment and adsorbed on the surface of AuNPs and as a result, the FAM is quenched by the AuNPs. Under optimized conditions, the presented fluorescent analytical approach showed high selectivity toward acrylamide with a wide linear response, 1 x 10(-7) M-0.05 M, and a limit of detection (LOD) of 1 x 10(-8) M for acrylamide. This new method indicated excellent analytical performance for acrylamide detection in potato fries water extract samples with LOD of 0.5 x 10(-8) M. (C) 2018 Elsevier B.V. All rights reserved.
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