Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 264, Issue -, Pages 320-326Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.02.159
Keywords
Lateral flow biosensor; microRNA; Simultaneous detection; Multiplex detection; Serum sample
Funding
- NSFC [21475030]
- S&T Research Project of Anhui Province [15czz03109]
- National 10000 Talents-Youth Top-notch Talent Program
- special fund of central university [2017HGPA0162]
- Science and Technology Ministry of China [2015BAD17B02-3]
- NIH, Centers of Biomedical Research Excellent (NIH, BOBRE) [P20 GM109024]
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The authors describe a rapid and low-cost approach for multiplex microRNA(miRNA) assay on lateral flow nucleic acid biosensor (LFNAB). The principle of assay is based on sandwich-type nucleic acid hybridization reactions to produce gold nanoparticle (GNP)-attached complexes (ssDNA-microRNA-ssDNA/GNPs), which are captured and visualized on the test zone of LFNAB. By designing three different test zones on LFNAB, simultaneous detection of microRNA-21, microRNA-155 and microRNA-210 was achieved with an adding-measuring model by using GNP as visual tag. The method was challenged by testing the microRNAs in spiked serum samples with satisfied results. In our perception, the test is a particularly valuable tool for clinical application and biomedical diagnosis, particularly in limited resource settings. (C) 2018 Elsevier B.V. All rights reserved.
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