Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 265, Issue -, Pages 498-505Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.03.094
Keywords
FMn-CDs; Ratiometric fluorescence biosensor; Dipicolinic acid; Visual FMn-CDs test paper
Funding
- Guangdong Natural Science Foundation [2017A030310652, 2017A030313255]
- Science and Technology Planning Project of Guangzhou City [201804010117]
- Science and Technology Planning Project of Guangdong Province [2011A060901013]
- Guangzhou University's training program for excellent new recruited doctors [YB201717]
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Functionalized manganese-doped carbon dots (FMn-CDs) were prepared by integration of pyrolysis derived Mn-CDs with ethylene diamine, ethylenediamine tetraacetic acid and Eu(III) ions. The FMn-CDs emitted intense blue fluorescence with high photostability, excellent water-solubility and favorable biocompatibility. 2, 6-dipicolinic acid (DPA) is an important biomarker of Bacillus anthracis spore. It serves as an antenna suction light group and can sensitize the Eu(III) combined on the FMn-CDs to produce bright red fluorescence. Benefitting from the absorbance energy transfer emission effect from DPA to the sensitized Eu(III), a ratiometric biosensor towards DPA detection was developed. The approach exhibited a linearity range from 0.1 to 750 nM and a detection limit of 0.1 nM. Moreover, visual FMn-CDs test paper was produced for the DPA detection using a smartphone under a portable UV lamp, with a lowest detection limit of 1 mu M. This work revealed the potential of a convenient smart phone diagnostics for DPA detection using visual FMn-CDs test paper. (C) 2018 Elsevier B.V. All rights reserved.
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