Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 254, Issue -, Pages 1133-1140Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.085
Keywords
Fluorescent RNA biosensor; Metal-organic framework; Dengue and Zika virus; Simultaneous detection; Single and synchronous analysis
Funding
- Guangdong Provincial Department of Science and Technology of China [2015A010105016]
- Natural Science Foundation of Guangdong [2015A030313284]
- Natural Science Foundation of China [21401098]
- open project of State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology) [MUST-SKL-2016-04]
- Macao Science and Technology Development Fund, Macau Special Administrative Region
- Tertiary Education Services Office, Macau Special Administrative Region
Ask authors/readers for more resources
A water soluble and stable metal-organic framework (MOF) [Cu(Dcbcp)(bpe)](n) (1, Dcbcp = N-(3,5-dicarboxylbenzyl)-(3-carboxyl) pyridinium, bpe = 1,2-bis(4-pyridyl) ethylene) was synthesized. MOF 1 possesses a three-dimensional open framework with large pores and has a high affinity toward carboxyfluorescein (FAM) or 5(6)-carboxyrhodamine, triethylammonium salt (ROX)-tagged single-stranded probe DNA (P-DNA) through pi stacking, electrostatic interactions, thus quenching the fluorescence of tag. The two formed P-DNA@1 systems can be applied for simultaneous detection of Dengue virus (DENV) and Zika virus (ZIKV) RNA sequences, which is attributed to the different affinities of MOF 1 with P-DNA and the double-stranded DNA/RNA formed upon recognition. With the same detection time of 36 min and 2 min, the detection limits are 332 and 192 pM with the single detection method, 184 and 121 pM with synchronous fluorescence detection method. Both assays are highly specific and not interfered by other mismatched RNA sequences, even down to single-base mismatched RNA sequences. There was no cross-reaction between the two probes for synchronous detection. Comparing these two methods, synchronous fluorescence analysis improves the detection efficiency by saving the time and increasing the detection limits, which may attribute to its avoiding the interference of Raleigh light scattering signal to the fluorescence signal. (C) 2017 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available