Facile method for enrofloxacin detection in milk using a personal glucose meter

We developed a simple method for enrofloxacin detection in water and milk using a personal glucose meter (PGM). After mixing a test sample with Escherichia coli (E. coli) in lysogeny broth (LB) and glucose, the amount of glucose consumed (change in concentration) by bacterial metabolism was measured using the PGM. The antibacterial activity of enrofloxacin obstructs bacterial metabolism and so reduces glucose consumption in proportion to the enrofloxacin concentration. The limit of detection for enrofloxacin in both matrices was 5 ng/mL and the assay time was 2 h. Further, the change in glucose concentration could be determined qualitatively by the naked eye using glucose test strips for high-throughput screening. (C) 2017 Elsevier B.V. All rights reserved.