4.7 Article

Multivalent glycosylated Cu:CdS quantum dots as a platform for rapid bacterial discrimination and detection

Journal

SENSORS AND ACTUATORS B-CHEMICAL
Volume 254, Issue -, Pages 431-436

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.07.130

Keywords

Bacterial discrimination; Quantum dots; Carbohydrate; Glycosylated; Linear discriminant analysis

Funding

  1. National Natural Science Foundation of China [41506094]
  2. National Key Basic Research Program of China [2014CB643304]
  3. Shandong Provincial Natural Science Foundation, China [ZR2014DQ009]
  4. Applied Basic Research Program of Qingdao [16-5-1-19-jch]

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In this work, a rapid bacterial discrimination and detection system based on multivalent glycosylated Cu:CdS quantum dots (QDs) was presented. Cu:CdS QDs with excellent fluorescent properties were synthesized via a facile one-step method, then three natural carbohydrates, glucose, stachyose and raffinose, were coupled onto the surface of Cu:CdS QDs to construct glycosylated platforms. This is the first time that glucose, stachyose and raffinose were used for construction bacterial detection and identification platform. Bacterial discrimination was realized by analyzing the difference of binding strength between glycosylated Cu:CdS QDs and bacterial cell walls. Linear discriminant analysis was used to discriminate the response patterns, and six different bacterial samples could be identified within 30 min. The greatest strengths of the bacterial discrimination platform based on multivalent glycosylated Cu:CdS QDs were rapid, simple and low-cost, there was no need for expensive reagents and instruments. (C) 2017 Elsevier B.V. All rights reserved.

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