Synthesis and chemosensing of nitrofurazone using olive oil based silver nanoparticles (O-AgNPs)

Silver nanoparticles were synthesized using Olive oil (O-AgNPs) as reducing as well as capping agent and extensively characterized by UV-vis spectroscopy, fourier transform infrared, energy disperse spectroscopy, dynamic light scattering and atomic force microscopy. The chrome yellow color solution of O-AgNPs show the typical absorption maximum at 430 nm. FTIR analysis revealed that the carbonyl (C=O) groups of Olive oil plays the most vital role in reduction of Ag+ and the nanoparticles synthesis. The morphology of O-AgNPs were found spherical in nature, while the size of O-AgNPs ranges between 35 to 65 nm as established by AFM and DLS studies, respectively. Stability of O-AgNPs studied by varying storage period, temperature, salt and pH of the medium were found quite stable probably because of oil suspension nature. Despite potent antimicrobial, anti-biofilm and biofilm eradicating activities, O-AgNPs were found to be non-toxic on cell lines. Moreover, chemosensing properties of the O-AgNPs were also tested against different drugs. The O-AgNPs showed high selectivity towards nitrofurazone (NFZ) with the lowest detection limit of 1.88 mu M as was monitored by UV-vis spectroscopy. The mechanism of O-AgNPs interaction with drug was followed by DLS suggest that NFZ induces a time dependent nanoparticles aggregation. (C) 2017 Elsevier B.V. All rights reserved.