Journal
INNOVATIVE RESEARCHES ON ALGAL BIOMASS
Volume 15, Issue -, Pages 43-46Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.proenv.2012.05.008
Keywords
squalene; octadecylbenzene; HPLC; Aurantiochytrium
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Squalene was determined by HPLC using octadecylbenzene (ODB) as an internal standard. Squalene and ODB were monitored at 210 nm. The retention times of squalene and ODB were 7.59 and 8.54 min, respectively. Squalene was determined from the peak area ratios of squalene/ODB detected at 210 nm. After treatment with 0.5 M KOH containing ethanol at 90 degrees C for 1 h, squalene in the saponified lipid fraction was extracted using n-hexane. No interfering peak was observed. Linearity of this method was observed in the range 80 900 ng. ODB is useful as an internal standard for squalene determinations. (C) 2012 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of International Organizing Committee for AOAIS-1
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