Journal
SENSORS
Volume 18, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/s18020385
Keywords
peptide; GO; ariginines; sensitivity; selectivity
Funding
- Natural Science Foundation of China [21302108]
- Shenzhen Municipal Government [JCYJ20160301153959476, JCYJ20160324163734374]
- Shenzhen Reform Commission (Disciplinary Development Program for Chemical Biology)
- China Scholarship Council
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The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.
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