Allelopathy and preliminary separation of allelopathic substance in the extracts of wheat seed germination

One variety of durum wheat (Triticum durum L.), 'Longfu 30', was choosen to study the allelopathic effects of seed germination extracts on other plants. The allelopathic effects of different polarity extracts were measured in terms of germination rate and germination speed on a test cucumber variety 'Jinyou No.2' (Cucumis sativus L.), and the allelopathic substance in chloroform extract was preliminarily separated with HSCCC and determined by GC-MS. The results showed that the allelopathy of wheat seed germination extracts varied with the polarity of solvent, and the substance in chloroform phase was the most active and showed a significantly inhibitory effect on seed germination of cucumber by 80.7% under Petri dish culture. The major allelopathic substance in wheat seed germination extracts were phenol, amine, piperazine, alcohols, esters and ketones.