4.7 Article

Seasonal distribution of nitrifiers and denitrifiers in urban river sediments affected by agricultural activities

Journal

SCIENCE OF THE TOTAL ENVIRONMENT
Volume 642, Issue -, Pages 1282-1291

Publisher

ELSEVIER
DOI: 10.1016/j.scitotenv.2018.06.116

Keywords

River sediments; Agricultural soils; Seasonal variation; Nitrifying gene; Denitrifying gene; Annamox; Feammox

Funding

  1. National Key Research and Development Program of China [2016YFE0106600]
  2. National Natural Science Foundation of China [41501278]
  3. Science and Technology Program by Guangdong Financial Department [PM-zx097-201601-024]
  4. Natural Science Foundation of Guangdong Province [2016A030313020]
  5. Science and Technology Program of Guangzhou, China [2016201604030057]
  6. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (Sun Yat-sen University) [2018K10]

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Nitrifiers and denitrifiers play a critical role in nitrogen removal in urban river sediments that are also affected by agricultural activities. However, the seasonal variations and vertical profile of these organisms in these river sediments are not well understood. In this study, the seasonal and depth (0 to 30 cm) distributions of the abundance and activity of nitrifiers and denitrifiers in sediments of the Pearl River in Guangzhou city were quantifying via qPCR and RT-qPCR according to various nitrifying and denitrifying functional genes, and their diversities were analyzed via high-throughput sequencing on an Illumina MiSeq platform. Results show that the distribution of nitrifiers and denitrifiers in these urban sediments were more abundant and active during the summer than winter; had distinct vertical distributions in the bacterial numbers and activity, with higher activity of the nirS gene (yearly averaged RNA:DNA 2.5% at 18 to 22 cm, vs. a yearly-depth average of 0.65%) but with lower overall numbers (yearly averaged 2.1 x 10(6) copies g(-1 )at 18 to 22 cm, vs. a yearly-depth average of 12.5 x 10(6 )copies g(-1)); and their amoA and nosZ gene diversities in the sediments exhibited a correlation with the communities in nearby agricultural soils. (C) 2018 Elsevier B.V. All rights reserved.

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