A highly reusable MANAE-agarose-immobilized Pleurotus ostreatus laccase for degradation of bisphenol A

Bisphenol A (BPA) is an endocrine disruptor compound that is continuously released into the environment and is barely degraded in wastewater treatment plants. A previous study showed that free Pleurotus ostreatus laccase is efficient in degrading BPA producing less toxic metabolites. In the present study, this laccase was successfully immobilized onto MANAE-agarose, improving its efficiency in degrading BPA and its thermal and storage stabilities. In addition to this, the immobilized enzyme retained >90% of its initial capability to degrade BPA after 15 cycles of reuse. P. ostreatus laccase immobilized onto MANAE-agarose could be an economical alternative for large scale degradation of BPA in aqueous systems. (C) 2018 Elsevier B. V.All rights reserved.