Journal
SCIENCE
Volume 361, Issue 6400, Pages 412-415Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aar4199
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Funding
- NIH director's New Innovator award [DP2CA195769]
- Pew Charitable Trusts through the Pew Biomedical Scholars Program grant
- NIH 4D Nucleome through NOFIC
- German Research Foundation (DFG) [SP1680/1-1]
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Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Poi II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator. recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.
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