Journal
SCIENCE
Volume 359, Issue 6378, Pages 935-939Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaq1067
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Funding
- Japan Ministry of Education, Culture, Sports, Science, and Technology [15H05948, 16H01426, 16H04663, 17K19451, 24650633]
- Adaptive, Seamless Technology Transfer Program through target-driven RD JST [AS2614119N]
- Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS)
- Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST)
- Uehara Memorial Foundation
- Special Postdoctoral Researchers Program from RIKEN
- Grants-in-Aid for Scientific Research [16H01426, 17K19451, 17K13272, 15H05948, 16H04663, 24650633] Funding Source: KAKEN
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Bioluminescence is a natural light source based on luciferase catalysis of its substrate luciferin. We performed directed evolution on firefly luciferase using a red-shifted and highly deliverable luciferin analog to establish AkaBLI, an all-engineered bioluminescence in vivo imaging system. AkaBLI produced emissions in vivo that were brighter by a factor of 100 to 1000 than conventional systems, allowing noninvasive visualization of single cells deep inside freely moving animals. Single tumorigenic cells trapped in the mouse lung vasculature could be visualized. In the mouse brain, genetic labeling with neural activity sensors allowed tracking of small clusters of hippocampal neurons activated by novel environments. In a marmoset, we recorded video-rate bioluminescence from neurons in the striatum, a deep brain area, for more than 1 year. AkaBLI is therefore a bioengineered light source to spur unprecedented scientific, medical, and industrial applications.
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