Journal
REPRODUCTIVE TOXICOLOGY
Volume 76, Issue -, Pages 26-34Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2017.12.002
Keywords
Leydig cell; Bisphenol A; Testosterone; Cell death; Metabolically active mitochondria
Categories
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- CNPQ (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- FINEP (Financiadora de Estudos e Projetos)
- Fundacao Araucaria
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Leydig cells are the major testosterone-producing cells of the male reproductive system, and damage to these cells can impair fertility of men. Bisphenol A (BPA) is one of the chemicals with the highest volume of production worldwide. The aim of this study was to evaluate the effects of BPA on the growth, viability, and testosterone production of TM3 murine Leydig cells after exposure to BPA for 24 or 48 h. BPA reduced testosterone production, cell viability and cell growth in a concentration-dependent manner. The highest tested concentration of BPA (100 mu M) increased cellular death, as indicated by an increased sub-G1 phase population and a larger number of cells labeled with Hoechst 3342. This concentration of BPA also decreased the number of metabolically active mitochondria as revealed by rhodamine staining. Therefore, our data show that BPA is toxic to Leydig TM3 cells and impairs their steroidogenic function. (C) 2017 Elsevier Inc. All rights reserved.
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