Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 30, Issue 11, Pages 1503-1513Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD17332
Keywords
activation; apoptosis; IGF-1; organ culture; preantral follicle
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Funding
- Science and Technology Foundation of Pernambuco state (FACEPE)
- National Council for Scientific and Technological Development (CNPq)
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We investigated the effects of insulin-like growth factor 1 (IGF-1) on the morphology and follicular activation of ovine preantral follicles cultured in situ and whether the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway is involved in IGF-1 action in the sheep ovary. Ovine ovarian fragments were fixed for histological and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) analyses (fresh control) or cultured in supplemented alpha-minimum essential medium (alpha-MEM+; control) or alpha-MEM+ with IGF-1 (1, 10, 50,100 or 200 ng mL(-1)) for 7 days. Follicles were classified as normal or atretic, primordial or growing and the oocyte and follicle diameters were measured. DNA fragmentation was evaluated by TUNEL assay. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was performed on the fresh control, alpha-MEM+ and 100 ng mL(-1) IGF-1 samples. Inhibition of PI3K activity was performed through pretreatment with the PI3K inhibitor LY294002 and phosphorylated AKT (pAKT) expression was analysed after culture in the absence or presence of LY294002. IGF-1 at 100 ng mL(-1) increased (P < 0.05) follicular activation compared with alpha-MEM+ and decreased TUNEL-positive cells (P < 0.05) compared with other treatments. PCNA-positive cells also increased (P < 0.05) in 100 ng mL(-1) IGF-l. LY294002 significantly inhibited follicular activation stimulated by alpha-MEM+ and 100 ng mL(-1) IGF-1 and reduced pAKT expression in follicles. Overall, IGF-1 at 100 ng mL(-1) promoted primordial follicle activation, cell proliferation and reduced DNA fragmentation after in situ culture through the PI3K/AKT pathway.
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