Journal
REDOX REPORT
Volume 23, Issue 1, Pages 125-129Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13510002.2018.1456000
Keywords
Oxidation-reduction potential; oxidative stress; plasma
Categories
Funding
- NIH [RO1 GM123010, UM1 HL120877]
- Aytu BioScience, Inc.
Ask authors/readers for more resources
Objectives: Oxidation-reduction potential (ORP) measurement can demonstrate the extent of oxidative stress in patients with severe illness and/or injury. A novel ORP diagnostic platform using disposable sensors (RedoxSYS) has been validated by comparison to mass spectrometry, but the optimal methods of sample handling for best performance of the device have not been described.Methods: We sought to optimize ORP measurement in human plasma under controlled conditions. We hypothesized that the anticoagulant, freeze-thawing, and storage duration would influence measured ORP levels.Results: The platform was sensitive to exogenous oxidation with hydrogen peroxide and reduction with ascorbic acid. Plasma anticoagulated with heparin was more sensitive to differences in ORP than plasma prepared in citrate. ORP measurements decreased slightly after a freeze-thaw cycle, but once frozen, ORP was stable for up to one month.Discussion: We confirm that ORP detects oxidative stress in plasma samples. Optimal measurement of plasma ORP requires blood collection in heparin anticoagulant tubes and immediate analysis without a freeze-thaw cycle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available