Journal
PROTOPLASMA
Volume 255, Issue 6, Pages 1827-1837Publisher
SPRINGER WIEN
DOI: 10.1007/s00709-018-1275-4
Keywords
Plasma membrane (PM) H+-ATPase; Salt tolerance; Ion homeostasis; Transgenic Arabidopsis; Sesuvium portulacastrum
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Funding
- Natural Science Foundation of China [31660253, 31260218]
- Scientific and Technological Foundation of Hainan Province [HNGDhs201502]
- Hainan Provincial Natural Science Foundation of China [318QN189]
- National Key Technology Support Program [2015BAD01B02]
- Hainan University [KYQD(ZR)1845]
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The SpAHA1 gene, encoding a plasma membrane (PM) H+-ATPase (AHA) in Sesuvium portulacastrum, was transformed into Arabidopsis plants, and its expression increased salinity tolerance of transgenic Arabidopsis plants: seed germination ratio, root growth, and biomass of transgenic plants were greater compared to wild-type plants under NaCl treatment condition. Upon salinity stress, both Na+ and H+ effluxes in the roots of SpAHA1 expressing plants were faster than those of untransformed plants. Transformed plants with SpAHA1 had lower Na+ and higher K+ contents relative to wild-type plants when treated with NaCl, resulting in greater K+/Na+ ratio in transgenic plants than in wild-type plants under salt stress. Extent of oxidative stress increased in both transgenic and wild-type plants exposed to salinity stress, but overexpression of SpAHA1 could alleviate the accumulation of hydrogen peroxide (H2O2) induced by NaCl treatment in transgenic plants relative to wild-type plants; the content of malondialdehyde (MDA) was lower in transgenic plants than that in wild-type plants under salinity stress. These results suggest that the higher H+-pumping activity generated by SpAHA1 improved the growth of transgenic plants via regulating ion and reactive oxygen species (ROS) homeostasis in plant cells under salinity stress.
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