Journal
PROCESS BIOCHEMISTRY
Volume 69, Issue -, Pages 1-11Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2018.03.005
Keywords
Ligninolytic enzymes; Bioreactor; Submerged cultivation; Experimental design; Biorefinery
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Funding
- Coordination Of Improvement of Higher Education Personnel (CAPES) [3255/2013]
- Research Support Foundation of the State of Rio Grande do Sul [16/2551-0000 246-0]
- Brazilian Agricultural Research Corporation (EMBRAPA) [34/2014]
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The present work was carried out to determine the best sources and concentrations of carbon and nitrogen in the production of laccases, total peroxidases and manganese peroxidase by Marasmiellus palmivorus VE111. Among the carbon and nitrogen sources used, the combination of glucose and casein led to higher production of laccases (5134 U/mL), total peroxidases (187 U/mL) and manganese peroxidase (57 U/mL). Central composite rotatable design (CCRD) was used to determine the optimum concentrations of glucose and casein to produce enzymes. The experiments with better results were scaled up in a 5 L bioreactor. It was verified that lower concentrations of glucose (5 and 10 g/L) stimulated higher activity of total peroxidases (1285 U/mL) and laccases (3420 U/mL), respectively, while a higher concentration of glucose (30 g/L) favoured the production of manganese peroxidase (59 U/mL). These data indicate high performance of M. palmivorus VE111 in the production of ligninolytic enzymes.
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