Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 6, Pages 1388-1393Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1715556115
Keywords
receptor-like kinase; plasmodesmata; RNAi; BAM1; Geminivirus
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Funding
- Centre of Excellence for Plant and Microbial Sciences (CEPAMS)
- UK Biotechnology and Biological Sciences Research Council
- Chinese Academy of Sciences
- Mianshang grant from the National Natural Science Foundation of China (NSFC) [31671994]
- Shanghai Center for Plant Stress Biology of the Chinese Academy of Sciences
- 100 Talent program of the Chinese Academy of Sciences
- President's International Fellowship Initiative (PIFI) postdoctoral fellowship from the Chinese Academy of Sciences [2016PB042]
- CAS-TWAS President's Fellowship
- BBSRC [BB/L000466/1, BB/J020222/1, BBS/E/J/000PR9797] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000PR9797, BB/L000466/1] Funding Source: researchfish
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RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an antiviral immune response. How this cell-to-cell spread of silencing is regulated is currently unknown. Here, we describe that the C4 protein from Tomato yellow leaf curl virus can inhibit the intercellular spread of RNAi. Using this viral protein as a probe, we have identified the receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) as a positive regulator of the cell-to-cell movement of RNAi, and determined that BAM1 and its closest homolog, BAM2, play a redundant role in this process. C4 interacts with the intracellular domain of BAM1 and BAM2 at the plasma membrane and plasmodesmata, the cytoplasmic connections between plant cells, interfering with the function of these RLKs in the cell-to-cell spread of RNAi. Our results identify BAM1 as an element required for the cell-to-cell spread of RNAi and highlight that signaling components have been coopted to play multiple functions in plants.
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