Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 10, Pages E2220-E2228Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1714582115
Keywords
biofilms; porins; intercellular communication; cell adhesion; steric zippers
Categories
Funding
- French Infrastructure for Integrated Structural Biology [ANR-10-INSB-05-02]
- Grenoble Alliance for Integrated Structural Cell Biology (GRAL) within the Grenoble Partnership for Structural Biology [ANR-10-LABX-49-01]
- Rhone-Alpes Region
- Fondation de la Recherche Medicale
- Fonds Europeen de Developpement Regional
- CNRS
- CEA
- UGA
- EMBL
- Groupement d'Interet Scientifique-Infrastrutures en Biologie, Sante et Agronomie
- ESRF [MX722, MX1464, MX1583]
- Agence Nationale de la Recherche Grant [ANR-15-CE18-0005-02]
- GRAL Grant [C7H-LXG11A20-COLLETIER]
- Laboratory of Excellence Ion Channel Science and Therapeutics Grant [ANR-11-LABX-0015-01]
- Aix-Marseille University
- Service de Sante des Armees
- CEA-GRAL doctoral fellowship [C7H-LXG11A20-DYNAMOP]
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The gram-negative pathogen Providencia stuartii forms floating communities within which adjacent cells are in apparent contact, before depositing as canonical surface-attached biofilms. Because porins are the most abundant proteins in the outer membrane of gram-negative bacteria, we hypothesized that they could be involved in cell-to-cell contact and undertook a structure-function relationship study on the two porins of P. stuartii, Omp-Pst1 and Omp-Pst2. Our crystal structures reveal that these porins can selfassociate through their extracellular loops, forming dimers of trimers (DOTs) that could enable cell-to-cell contact within floating communities. Support for this hypothesis was obtained by studying the porin-dependent aggregation of liposomes and model cells. The observation that facing channels are open in the two porin structures suggests that DOTs could not only promote cellto-cell contact but also contribute to intercellular communication.
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