Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 5, Pages E1012-E1021Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1706928115
Keywords
antigen presentation; epigenetics; coronavirus; influenza; DNA methylation
Categories
Funding
- NIH National Institute of Allergy and Infectious Diseases [U19AI100625, U19AI106772]
- NIH National Institute of Aging [K99AG049092]
- Department of Energy (DOE) Office of Biological and Environmental Research
- National Institute of General Medical Sciences Proteomics Research Resource [GM103493]
- DOE [DE-AC05-76RLO1830]
- Grants-in-Aid for Scientific Research [16H06434, 16H06429] Funding Source: KAKEN
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Convergent evolution dictates that diverse groups of viruses will target both similar and distinct host pathways to manipulate the immune response and improve infection. In this study, we sought to leverage this uneven viral antagonism to identify critical host factors that govern disease outcome. Utilizing a systems-based approach, we examined differential regulation of IFN-gamma-dependent genes following infection with robust respiratory viruses including influenza viruses [A/influenza/Vietnam/1203/2004 (H5N1-VN1203) and A/influenza/California/04/2009 (H1N1-CA04)] and coronaviruses [severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV)]. Categorizing by function, we observed down-regulation of gene expression associated with antigen presentation following both H5N1-VN1203 and MERS-CoV infection. Further examination revealed global down-regulation of antigen-presentation gene expression, which was confirmed by proteomics for both H5N1-VN1203 and MERS-CoV infection. Importantly, epigenetic analysis suggested that DNA methylation, rather than histone modification, plays a crucial role in MERS-CoV-mediated antagonism of antigen-presentation gene expression; in contrast, H5N1-VN1203 likely utilizes a combination of epigenetic mechanisms to target antigen presentation. Together, the results indicate a common mechanism utilized by H5N1-VN1203 and MERS-CoV to modulate antigen presentation and the host adaptive immune response.
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