Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 8, Pages E1906-E1915Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1712251115
Keywords
Arabidopsis; BRI1; ubiquitination; E3 ligase; endocytosis
Categories
Funding
- NSF [IOS-1252539]
- NIH [R01GM097247]
- Robert A. Welch Foundation [A-1795]
- Shanghai Sailing Program Grant [17YF1406400]
- Research Foundation-Flanders
- Bulgarian Academy of Sciences Grant [VS.025.13N]
- Belgian Science Policy Office
- [QD2016035]
- [BOF15/24J/048]
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Plants largely rely on plasma membrane (PM)-resident receptor-like kinases (RLKs) to sense extracellular and intracellular stimuli and coordinate cell differentiation, growth, and immunity. Several RLKs have been shown to undergo internalization through the endocytic pathway with a poorly understood mechanism. Here, we show that endocytosis and protein abundance of the Arabidopsis brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), are regulated by plant U-box (PUB) E3 ubiquitin ligase PUB12-and PUB13-mediated ubiquitination. BR perception promotes BRI1 ubiquitination and association with PUB12 and PUB13 through phosphorylation at serine 344 residue. Loss of PUB12 and PUB13 results in reduced BRI1 ubiquitination and internalization accompanied with a prolonged BRI1 PM-residence time, indicating that ubiquitination of BRI1 by PUB12 and PUB13 is a key step in BRI1 endocytosis. Our studies provide a molecular link between BRI1 ubiquitination and internalization and reveal a uniquemechanism of E3 ligase-substrate association regulated by phosphorylation.
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