Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 18, Pages E4189-E4198Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1719010115
Keywords
estrogen receptor alpha; transcriptome; DNA methylation; mouse seminal vesicle; neonatal DES exposure
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Funding
- National Institute of Environmental Health Sciences (NIEHS)/NIH Comparative Medicine Branch
- Intramural Research Division of the NIEHS [1ZIAES70065, 1ZIAES102985]
- Pathology Core facility for the LCM study
- Epigenetic Core facility for providing MBD-Seq and TBS-Seq
- NIH Intramural Sequencing Center for providing RNA-Seq data
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Early transient developmental exposure to an endocrine active compound, diethylstilbestrol (DES), a synthetic estrogen, causes late-stage effects in the reproductive tract of adult mice. Estrogen receptor alpha (ER alpha) plays a role in mediating these developmental effects. However, the developmental mechanism is not well known in male tissues. Here, we present genome-wide transcriptome and DNA methylation profiling of the seminal vesicles (SVs) during normal development and after DES exposure. ER alpha mediates aberrations of the mRNA transcriptome in SVs of adult mice following neonatal DES exposure. This developmental exposure impacts differential diseases between male (SVs) and female (uterus) tissues when mice reach adulthood due to most DES-altered genes that appear to be tissue specific during mouse development. Certain estrogen-responsive gene changes in SVs are cell-type specific. DNA methylation dynamically changes during development in the SVs of wild-type (WT) and ER alpha-knockout (alpha ERKO) mice, which increases both the loss and gain of differentially methylated regions (DMRs). There are more gains of DMRs in alpha ERKO compared with WT. Interestingly, the methylation changes between the two genotypes are in different genomic loci. Additionally, the expression levels of a subset of DES-altered genes are associated with their DNA methylation status following developmental DES exposure. Taken together, these findings provide an important basis for understanding the molecular and cellular mechanism of endocrine-disrupting chemicals (EDCs), such as DES, during development in the male mouse tissues. This unique evidence contributes to our understanding of developmental actions of EDCs in human health.
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