Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 9, Pages 2090-2095Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1716161115
Keywords
bovine; embryonic stem cell; pluripotency; inner cell mass
Categories
Funding
- NIH/Eunice Kennedy Shriver National Institute of Child Health and Human Development [R01-HD070044]
- United States Department of Agriculture (USDA)-National Institute of Food and Agriculture (NIFA)-Agriculture and Food Research Initiative (AFRI) [W3171]
- University of California, Davis Academic Senate New Research Grant
- National Science Foundation [1148897]
- Sao Paulo Research Foundation (FAPESP) [2013/08135-2, 2015/25111-5]
- Universidad Catolica San Antonio de Murcia
- G. Harold and Leila Y. Mathers Charitable Foundation
- Moxie Foundation
- Fundacion Dr. Pedro Guillen
- NIH [DP1-DK113616]
- Grants-in-Aid for Scientific Research [16H04796] Funding Source: KAKEN
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Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.
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