Journal
OPTICS EXPRESS
Volume 21, Issue 4, Pages 5164-5170Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.21.005164
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Funding
- Rowland Junior Fellows program
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Imaging fluorescence in moving cells is fundamentally challenging because the exposure time is constrained by motion-blur, which limits the available signal. We report a method to image fluorescently labeled leukemia cells in fluid flow that has an effective exposure time of up to 50 times the motion-blur limit. Flowing cells are illuminated with a pseudo-random excitation pulse sequence, resulting in a motion-blur that can be computationally removed to produce near diffraction-limited images. This method enables observation of cellular organelles and their behavior in a fluid environment that resembles the vasculature. (C) 2013 Optical Society of America
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