Journal
JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
Volume 52, Issue 1, Pages 17-21Publisher
AMER ASSOC LABORATORY ANIMAL SCIENCE
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Funding
- Office of the Director, NIH [2R44RR025737-02, 8R44OD010958]
- Kentucky Cabinet for Economic Development, Office of Commercialization and Innovation [KSTC-184-512-11-115]
- Kentucky Science and Technology Corporation
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The use of a murine nonsurgical embryo transfer (NSET) device had been described previously for the transfer of blasto-cysts, morulae, DNA-microinjected embryos, and embryonic stem cell-containing embryos to create genetically modified mice. However, physiologic effects of the NSET device and traditional surgical methods had not been compared directly. Here we used electrocardiography and fecal corticosterone levels to monitor pseudopregnant mice that underwent anesthesia only, the NSET procedure with or without anesthesia, or surgery. These procedures were performed without the use of actual embryos, to focus on effects of the procedures themselves rather than on any physiologic effects due to the deposition of embryos. As compared with surgery and anesthesia, the NSET procedure was associated with less fluctuation in cardiac rhythm and lower levels of the stress biomarker fecal corticosterone. These results indicate that use of the NSET device avoids these physiological perturbations as well as other disadvantages of surgery (for example, postoperative pain and need for postoperative analgesia) and therefore provides a valuable refinement of existing mouse embryo transfer procedures.
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