Journal
PLANT SCIENCE
Volume 267, Issue -, Pages 20-31Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2017.11.005
Keywords
Grapevine (Vitis vinifera); Powdery mildew (Erysiphe necator); H2O2 accumulation; Extrahaustorial membrane; Haustorial complex; Cell death
Categories
Funding
- National Natural Science Foundation of China [31372022, 31071772]
- Division Of Integrative Organismal Systems [1457033] Funding Source: National Science Foundation
Ask authors/readers for more resources
Powdery mildew is the most economically important disease of cultivated grapevines worldwide. Here, we report that the Arabidopsis broad-spectrum disease resistance gene RPW8.2 could improve resistance to powdery mildew in Vitis vinifera cv. Thompson Seedless. The RPW8.2-YFP fusion gene was stably expressed in grapevines from either the constitutive 35S promoter or the native promoter (NP) of RPW8.2. The grapevine shoots and plantlets transgenic for 35S::RPW8.2-YFP showed reduced rooting and reduced growth at later development stages in the absence of any pathogens. Infection tests with an adapted grapevine powdery mildew isolate En NAFU1 showed that hyphal growth and sporulation were significantly restricted in transgenic grapevines expressing either of the two constructs. The resistance appeared to be attributable to the ectopic expression of RPW8.2, and associated with the enhanced encasement of the haustorial complex (EHC) and onsite accumulation of H2O2. In addition, the RPW8.2-YFP fusion protein showed focal accumulation around the fungal penetration sites. Transcriptome analysis revealed that ectopic expression of RPW8.2 in grapevines not only significantly enhanced salicylic acid-dependent defense signaling, but also altered expression of other phytohormofie-associated genes. Taken together, our results indicate that RPW8.2 could be utilized as a trans gene for improving resistance against powdery mildew in grapevines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available