4.5 Article

Rapid detection of Fusarium oxysporum f. sp lactucae on soil, lettuce seeds and plants using loop-mediated isothermal amplification

Journal

PLANT PATHOLOGY
Volume 67, Issue 7, Pages 1462-1473

Publisher

WILEY
DOI: 10.1111/ppa.12855

Keywords

forma specialis; fusarium wilt; Lactuca sativa; loop-mediated isothermal amplification; seedborne pathogen; soilborne pathogen

Funding

  1. European Union [634179]

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Fusarium oxysporum f. sp. lactucae (FOL) is a soil- and seedborne pathogen and the causal agent of fusarium wilt on lettuce. Four races have been identified within FOL, with different worldwide distribution. Several molecular techniques have been used to detect and identify this pathogen; however, not all of them have the optimal characteristics in terms of sensitivity to perform FOL detection in plant and seed material. A loop-mediated isothermal amplification (LAMP) assay was developed based on the sequence-characterized amplified region (SCAR) obtained in a previous rapid amplification of polymorphic DNA (RAPD) study. The LAMP assay has been validated according to the EPPO standard PM7/98. The LAMP assay was tested with lettuce seeds, soil and plant material, and can be used successfully to amplify DNA from each of these matrices. In seed lots artificially inoculated with FOL, the detection limit of the LAMP test was 0.004% infected seed.

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