Journal
PLANT CELL
Volume 30, Issue 1, Pages 134-152Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.17.00626
Keywords
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Funding
- Alexander von Humboldt Foundation
- NSF CAREER award [MCB-1552455]
- NIH-NIGMS [R35GM124806, R01GM114292]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1552455] Funding Source: National Science Foundation
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Ribosome biogenesis is a fundamental process required for all cellular activities. Histone deacetylases play critical roles in many biological processes including transcriptional repression and rDNA silencing. However, their function in pre-rRNA processing remains poorly understood. Here, we discovered a previously uncharacterized role of Arabidopsis thaliana histone deacetylase HD2C in pre-rRNA processing via both canonical and noncanonical manners. HD2C interacts with another histone deacetylase HD2B and forms homo- and/or hetero-oligomers in the nucleolus. Depletion of HD2C and HD2B induces a ribosome-biogenesis deficient phenotype and aberrant accumulation of 18S pre-rRNA intermediates. Our genome-wide analysis revealed that HD2C binds and represses the expression of key genes involved in ribosome biogenesis. Using RNA immunoprecipitation and sequencing, we further uncovered a noncanonical mechanism of HD2C directly associating with pre-rRNA and small nucleolar RNAs to regulate rRNA methylation. Together, this study reveals a multifaceted role of HD2C in ribosome biogenesis and provides mechanistic insights into how histone deacetylases modulate rRNA maturation at the transcriptional and posttranscriptional levels.
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