Journal
PLANT BREEDING
Volume 137, Issue 2, Pages 203-209Publisher
WILEY
DOI: 10.1111/pbr.12563
Keywords
Aphis craccivora; backcrossing; cowpea; marker-assisted selection; polymorphism; Vigna unguiculata
Funding
- United States Agency for International Development [EDH-A-00-07-00005-00]
- Kirkhouse Trust
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The utility of combining simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) marker genotyping was determined for genetically mapping a novel aphid (Aphis craccivora) resistance locus in cowpea breeding line SARC 1-57-2 and for introgressing the resistance into elite cultivars by marker-assisted backcrossing (MABC). The locus was tagged with codominant SSR marker CP 171F/172R with a recombination fraction of 5.91% in an F-2 population from Apagbaala' x SARC 1-57-2. A SNP-genotyped biparental recombinant inbred line population was genotyped for CP 171F/172R, which was mapped to position 11.5 cM on linkage group (LG) 10 (physical position 30.514Mb on chromosome Vu10). Using CP 171F/172R for foreground selection and a KASP-SNP-based marker panel for background selection in MABC, the resistance from SARC 1-57-2 was introduced into elite susceptible cultivar Zaayura'. Five BC4F3 lines of improved Zaayura' that were isogenic except for the resistance locus region had phenotypes similar to SARC 1-57-2. This study identified a novel aphid resistance locus and demonstrated the effectiveness of integrating SSR and SNP markers for trait mapping and marker-assisted breeding.
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