4.7 Article

The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew

Journal

PLANT AND CELL PHYSIOLOGY
Volume 59, Issue 4, Pages 811-827

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcy020

Keywords

Blumeria graminis f. sp Hordei; Cuticular wax; Hordeum vulgare; beta-Ketoacyl-CoA synthase; Very-long-chain fatty acids

Funding

  1. National Natural Science Foundation of China [41621001, 31170369]
  2. Japanese Ministry of Agriculture, Forestry and Fisheries [TRS1002]
  3. Science and Technology Commission of Shanghai Municipality [14DZ2260400]
  4. Swiss National Science Foundation [31003A_146276, 31003A_170127]
  5. Danish Research Council [DOK2447104]
  6. Natural Sciences and Engineering Research Council of Canada
  7. Swiss National Science Foundation (SNF) [31003A_146276, 31003A_170127] Funding Source: Swiss National Science Foundation (SNF)

Ask authors/readers for more resources

The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, beta-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C-16-C-20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available