Chemical constituents of hemp (Cannabis sativa L.) seed with potential anti-neuroinflammatory activity

A new lignanamide (1) and a new coumaroylamino glycoside derivative (2) were isolated and identified from hemp (Cannabis sativa L.) seed along with eighteen known compounds (3-20), eight (9, 10, 14-18, 20) of which were isolated for the first time from hemp seed. Their anti-neuroinflammatory activity on LPS (lipopolysaccharide)-induced BV2 microglia cells was evaluated. Compounds 1, 2, 5-9, 12, 13, 15-17, 19 and 20 exhibited significant inhibitory effects on TNF-alpha release from LPS-induced BV2 microglia cells, especially the new glucoside 2. The results laid a solid foundation for additional research on hemp seed related to its value against neurodegenerative diseases.