Journal
FEMS MICROBIOLOGY REVIEWS
Volume 37, Issue 3, Pages 407-427Publisher
OXFORD UNIV PRESS
DOI: 10.1111/1574-6976.12015
Keywords
single-cell analysis; whole-genome amplification; multiple displacement amplification; microfluidics; microencapsulation; micromanipulation
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Funding
- NIH [R01 HG004863]
- Burroughs Wellcome Fund
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Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology.
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