Journal
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
Volume 22, Issue -, Pages 169-177Publisher
ELSEVIER
DOI: 10.1016/j.pdpdt.2018.04.011
Keywords
Manganese carbonate; Multilayered; Photodynamic therapy; Polyelectrolyte; Silicon(IV) phthalocyanine
Categories
Funding
- Foundation for the State of Sao Paulo Research - FAPESP project [2009/51729-5, 2013/50181-1, 01.10.0758.01]
- CNPq-National Institute of Science and Technology-INCT of Nanobiotechnology [573880/2008-5]
Ask authors/readers for more resources
Background: Microcapsules fabricated using layer-by-layer self-assembly have unique properties, making them attractive for drug delivery applications. The technique has been improved, allowing the deposition of multiple layers of oppositely charged polyelectrolytes on spherical, colloidal templates. These templates can be decomposed by coating multiple layers, resulting in hollow shells. In this paper, we describe a novel drug delivery system for loading photosensitizer drugs into hollow multilayered microcapsules for photoprocess applications. Methods: Manganese carbonate particles were prepared by mixing NH4HCO3 and MnSO4 and performing consecutive polyelectrolyte adsorption processes onto these templates using poly-(sodium 4-styrene sulfonate) and poly-(allylamine hydrocholoride). A photosensitizer was also incorporated into the layers. Hollow spheres were fabricated by removing the cores in the acidic solution. The hollow, multilayered microcapsules were studied by scanning electron microscopy, steady-state, and time-resolved techniques. Their biological activity was evaluated in vitro with cancer cells using a conventional MTT assay. Results: The synthesized CaCO3 microparticles were uniform, non-aggregated, and highly porous spheres. The phthalocyanine derivatives loaded in the microcapsules maintained their photophysical behaviour after encapsulation. The spectroscopic results presented here showed excellent photophysical behaviour of the studied drug. We observed a desirable increase in singlet oxygen production, which is favourable for the PDT protocol. Cell viability after treatment was determined and the proposed microcapsules caused 80% cell death compared to the control. Conclusions: The results demonstrate that photosensitizer adsorption into the CaCO3 microparticle voids together with the layer-by-layer assembly of biopolymers provide a method for the fabrication of biocompatible microcapsules for use as biomaterials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available