Journal
OPTICS LETTERS
Volume 43, Issue 8, Pages 1870-1873Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.43.001870
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Funding
- National Natural Science Foundation of China (NSFC) [61471313]
- Natural Science Foundation of Hebei Province [F2018203229]
- Qinhuangdao Science and Technology Bureau [201703A009]
- Yanshan University (YSU) Doctoral Funds [B1025]
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We propose a numerical and totally automatic phase aberration compensation method in digital holographic microscopy. The phase aberrations are extracted in a nonlinear optimization procedure in which the phase variation of the reconstructed object wave is minimized. Not only phase curvature but also high-order aberrations could be corrected without extra devices. The correction is directly carried out with the wrapped phase map, which is not affected by phase unwrapping or fitting errors. Numerical simulation proves that the proposed method is more accurate than the conventional surface fitting method without selecting a cell-free background. Experimental results demonstrate the availability of the proposed method in real-time analysis of living cells. (C) 2018 Optical Society of America
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