Journal
OPTICS EXPRESS
Volume 26, Issue 11, Pages 14499-14513Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.26.014499
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Funding
- Deutsche Forschungsgemeinschaft (DFG
- Emmy Noether Program)
- Deutsche Forschungsgemeinschaft (DFG
- CRC) [1101]
- European Research Council (ERC) [Nanomech 260875]
- Technische Universitat Dresden
- Universitat Tubingen
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Optical tweezers combined with various microscopy techniques are a versatile tool for single-molecule force spectroscopy. However, some combinations may compromise measurements. Here, we combined optical tweezers with total-internal-reflection-fluorescence (TIRF) and interference-reflection microscopy (IRM). Using a light-emitting diode (LED) for IRM illumination, we show that single microtubules can be imaged with high contrast. Furthermore, we converted the IRM interference pattern of an upward bent microtubule to its three-dimensional (3D) profile calibrated against the optical tweezers and evanescent TIRF field. In general, LED-based IRM is a powerful method for high-contrast 3D microscopy. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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