Journal
OPTICS EXPRESS
Volume 26, Issue 12, Pages 15759-15768Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.26.015759
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Funding
- National Natural Science Foundation of China (NSFC) [61635003, 61475020, 51535002]
- National Key R&D Program of China [2016YFF0201005]
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We present a correlation-differential confocal microscopy (CDCM), a novel method that can simultaneously improve the three-dimensional spatial resolution and axial focusing accuracy of confocal microscopy ( CM). CDCM divides the CM imaging light path into two paths, where the detectors are before and after the focus with an equal axial offset in opposite directions. Then, the light intensity signals received from the two paths are processed by the correlation product and differential subtraction to improve the CM spatial resolution and axial focusing accuracy, respectively. Theoretical analyses and preliminary experiments indicate that, for the excitation wavelength of lambda = 405 nm, numerical aperture of NA = 0.95, and the normalized axial offset of u(M) = 5.21, the CDCM resolution is improved by more than 20% and more than 30% in the lateral and axial directions, respectively, compared with that of the CM. Also, the axial focusing resolution important for the imaging of sample surface profiles is improved to 1 nm. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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