Journal
ONCOLOGY RESEARCH
Volume 26, Issue 2, Pages 241-247Publisher
COGNIZANT COMMUNICATION CORP
DOI: 10.3727/096504017X14953948675412
Keywords
Epithelial ovarian cancer (EOC); Long noncoding RNAs (lncRNAs); CCAT2; miR-424
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Funding
- Basic Medical Research Center of Nanjing Medical University
- Department of Gynecology, Huai'an First People's Hospital
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Epithelial ovarian cancer (EOC) is the one of most common gynecological malignant tumors with high mortality. A series of long noncoding RNAs (lncRNAs) have been validated to play a vital role in EOC tumorigenesis. Colon cancer-associated transcript 2 (CCAT2) has been verified as an oncogenic lncRNA in multiple tumors; however, the role of CCAT2 in FAX: genesis is still unclear. The purpose of the present study was to probe the function of CCAT2 on EOC. Preliminary experiments found that CCAT2 expression was significantly upregulated in EOC tissues and cell lines compared to noncancerous tissue and cells. CCAT2 knockdown induced by interfering oligonucleotides could inhibit proliferation and promote apoptosis and induce cell cycle arrest at the G(0)/G(1) phase. Bioinformatics analysis predicted that miR-424 targeted CCAT2, which was confirmed by luciferase reporter assay. Moreover, the miR-424 inhibitor rescued the tumorigenesis inhibition induced by CCAT2 knockdown. In summary, our findings illustrate that CCAT2 acts as competing endogenous RNA (ceRNA) or sponge via negatively targeting miR-424, providing a novel diagnostic marker and therapeutic target for EOC.
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